https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50167 Wed 05 Jul 2023 16:17:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36305 Tue 22 Jun 2021 12:32:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29100 Thu 26 Jul 2018 13:14:56 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29784 sgER) and the 5'-flanking region of the gene from the Sydney rock oyster Saccostrea glomerata. The sgER cDNA is predicted to encode a 477-amino acid protein that contains a DNA-binding domain (DBD) and a ligand-binding domain (LBD) typically conserved among both vertebrate and invertebrate ERs. A comparison of the sgER LBD sequence with those of other ligand-dependent ERs revealed that the sgER LBD is variable at several conserved residues known to be critical for ligand binding and receptor activation. Ligand binding assays using fluorescent-labelled E2 and purified sgER protein confirmed that sgER is devoid of estrogen binding. In silico analysis of the sgER 5'-flanking sequence indicated the presence of three putative estrogen responsive element (ERE) half-sites and several putative sites for ER-interacting transcription factors, suggesting that the sgER promoter may be autoregulated by its own gene product. sgER mRNA is ubiquitously expressed in adult oyster tissues, with the highest expression found in the ovary. Ovarian expression of sgER mRNA was significantly upregulated following in vitro and in vivo exposure to 17β-estradiol (E2). Notably, the activation of sgER expression by E2 in vitro was abolished by the specific ER antagonist ICI 182, 780. To determine whether sgER expression is epigenetically regulated, the in vivo DNA methylation status of the putative proximal promoter in ovarian tissues was assessed using bisulfite genomic sequencing. The results showed that the promoter is predominantly hypomethylated(with 0–3.3% methylcytosines) regardless of sgER mRNA levels. Overall, our investigations suggest thatthe estrogen responsiveness of sgER is regulated by a novel ligand-dependent receptor, presumably via a non-genomic pathway(s) of estrogen signalling.]]> Thu 14 Sep 2017 11:29:22 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1372 Sat 24 Mar 2018 08:28:20 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34582 Fri 29 Mar 2019 11:40:28 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34757 Fri 05 May 2023 15:48:50 AEST ]]>